Determination of Psoralen and Isopsoralen in Bushen Jiannao Soft Capsule by HPLC
- VernacularTitle:HPLC法测定补肾健脑软胶囊中补骨脂素和异补骨脂素的含量
- Author:
Xiaoming XU
;
Dayong XU
;
Junbo XING
- Publication Type:Journal Article
- Keywords:
Bushen Jiannao Soft Capsule;
psoralen;
isopsoralen;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of psoralen and isopsoralen in Bushen Jiannao Soft Capsule. Methods The HPLC separation was performed on Diamonsil-C18 (250 mm?4.6 mm, 5 ?m) column with a mixture methanol-water (48∶52) as the mobile phase, at a flow rate of 1.0 mL/min. Detection wavelength was at 246 nm, and column temperature at 40 ℃. Results The linear response of psoralen and isopsoralen ranged from 0.010 08～0.252 ?g (r=0.999 8) and 0.009 14～0.228 5 ?g (r =0.999 9). The average recovery was 98.91% (RSD=1.74%) and 99.93% (RSD=1.27%) respectively. Conclusion The method is simple, accurate, and suitable for the determination of the psoralen and isopsoralen in the preparations.
