Determination of Naringin and Hesperidin in Zengshi Keli by HPLC
- VernacularTitle:HPLC法测定增食颗粒中柚皮苷和橙皮苷的含量
- Author:
Zhaohui GUO
;
Shuchang WEI
;
Xiaomei OUYANG
- Publication Type:Journal Article
- Keywords:
Zengshi Keli;
HPLC;
Fructus Aurantii Immaturus;
Naringin;
Hesperidin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining the content of Naringin and Hesperidin in Zengshi Keli by HPLC. Methods The Kromasil C18 column with acetonitrile-water-phosphoric acid (20∶80∶0.02) as the mobile phase was used. The flow rate was 1.0 mL/min, the detective wavelength was 283 nm, the temperature of column is 35 ℃. Results The calibration curve were linear in the range of 0.178 5～0.892 5 ?g for Naringin and 0.073 68～0.368 4 ?g for Hesperidin (r=0.999 9) respectively. The average recovery was 97.24% (RSD=1.21%) and 96.95% (RSD=1.49%) respectively. Conclusion The method was simple, accurate, reproducible and can be used for quality control of Zengshi Keli.
