Determination of Silybin in Fufang Yiganling Tablet by HPLC
- VernacularTitle:HPLC法测定复方益肝灵片中水飞蓟宾的含量
- Author:
Haifeng GU
;
Xiaoqian ZHANG
;
Zengrong CHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Fufang Yiganling tablet;
Silybin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of silybin in Fufang Yiganling tablet. Methods RP-HPLC was used and the mobile phase was consisted of methanol-water-glacial acetic acid (48∶52∶1). The detection wavelength was 286 nm. Results The calibration curve of Silybin was linear in the range of 0.238 0～2.380 ?g, linear equation:Y=2.146?106X +1.818?103, r=0.999 9, with average recovery of 98.17% (RSD=1.3%). Conclusions The method is simple, accurate and with good reproducibility. It can be used to control the quality of silybin in Fufang Yiganling tablet.
