The biological character and fluorescent labeling of the fetal bone mesenchymal stem cells
- VernacularTitle:胎儿骨髓间充质干细胞生物学特征及荧光标记
- Author:
Jing TIAN
- Publication Type:Journal Article
- Keywords:
Foetus;
Bone mesenchymal stem cell;
Isolated culture;
DAPI
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the isolation,culture,proliferation and fluor-labelling of the fetal bone mesenchymal stem cell (BMSCs),and their biological character. Methods:Limb bone marrow samples from fetus aged 4 to 5 months were obtained, BMSCs were isolated by Ficoll centrifugalization and adherence screening method,and then cultured,proliferated to the 12th generation in vitro. The growth curve,cell cycle,phenotype,enzymatic activity,glycogen and the DAPI fluor-labelling of BMSCs were observed. Results:(1)Major BMSCs were fusiform, arranged as whirlpool. After the 12th generation,BMSCs presented the signs of senility. The subcultured cells proliferated faster than primary cells. (2)The 3rd and 8th generation of BMSCs expressed CD29,D71,but not CD34,HLA-DR;the G0,G1 period cells were 93.73%,and the S,M,G2 period cells were 6.27%. (3)The 5th generation cells were positive of nonspecific esterase and glycogen,but weak positive of alkaline phosphatase. (4)DAPI labeled BMSCs grew normaly,and the marking rate of BMSCs was 100%,but decreased gradually,and only 15% cells were labbelled for the 3th week. Conclusion:The acquired fetal BMSCs have the character of MSCs. The DAPI labeling is a modus operandi for the short period tracing BMSCs.
