Study of unification of endoplasmic reticulum and mitochondrium in fetal rat cerebral neuron apoptosis induced by intrauterine distress
- VernacularTitle:内质网应激联合线粒体在宫内窘迫胎鼠脑神经细胞凋亡中的作用研究
- Author:
Min LU
- Publication Type:Journal Article
- Keywords:
Intrauterine distress;
Apoptosis;
Endoplasmic reticulum;
Mitochondrium
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluae the unification of endoplasmic reticulum and mitochondrium in fetal rat cerebral neuron apoptosis induced by intrauterine distress. Methods:Fetal rat intrauterine distress model was established. The samples were randomly divided into normal group and ischemia-reperfusion group(IR). Expression of GRP78,caspase-9,caspase-12,and cytoron C protein were detected by western blot technique. Results:The expression of GRP78 on corresponding time in IR group was significantly higher than that in normal group. And it reached the maximum at 3h of reperfusion. The expression of GRP78 reduced step by step as reperfusion time extended. The expression of caspase-9 and caspase-12 at corresponding time in IR group were significantly higher than those in normal group. Their expression increased rapidly and reached the maximum at 12h of reperfusion. The expression of cytoron C in kytoplasm at corresponding time in IR group was significantly higher than that in normal group and reached the maximum at 12h of reperfusion. Conclusion:The synergistic effect of endoplasmic reticulum and mitochondrium plays an important role in fetal rat cerebral neuron apoptosis induced by intrauterine distress.