Construction of EGF coupling bovine serum albumin nano-carrier
- VernacularTitle:EGF偶联牛血清白蛋白纳米载体的构建
- Author:
Shubin WANG
- Publication Type:Journal Article
- Keywords:
EGF;
Bovine serum albumin;
Nano-carrier;
Copling
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare EGF-coupling bovine serum albumin nano-carrier as a new way for initiative targeted drug delivery of epidermal growth factor receptor(EGFR) with broad range of expression in tumor cell. Methods:albumin nano-carrier was prepared by second phacoemulsification and solvent evaporation technology,and epidermal growth factor(EGF) was conjugated with albumin nano-carrier by chemical crosslinking reagents for EGF coupling bovine serum albumin nano-carrier. Results:The prepared nano-carrier then underwent isotope labeling,then the coupling effect of EGF and its coupling level were certified by sephadex column and polyacrylamide gel electrophoresis(SDS-PAGE). Conclusion:Success in the preparation of the smaller bovine serum albumin nano-carrier and EGF coupling bovine serium albumin nano-carrier establish the experimental foundation for further exploration of its targeting of tumor cells in vitro and distribution in vivo.
