Effect of DHAQ-GM-CSF immunoliposomes on HL60 cells
- VernacularTitle:DHAQ-GM-CSF免疫脂质体对HL60细胞的杀伤作用研究
- Author:
Ying ZHANG
- Publication Type:Journal Article
- Keywords:
Immunoliposomes;
Targeted immunological therapy;
Leukemia;
HL60 cells
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare DHAQ-GM-CSF immunoliposomes and observe the cytotoxicity of HL60 cells treated with the immunoliposomes for providing the preclinical data for AML therapy. Methods:DHAQ liposomes were prepared by reverse-phase evaporation(REV),and GM-CSF was coupled to the DHAQ liposomes.The cytotoxicity of HL60 cells treated with the immunoliposomes was measured by tetrazolium microculture(MTT) celluar cytotoxicity test.The data of cellar untrastructure and apoptotic rate were collected by transmission electron microscopy,and flow cytometric analysis. Results:DHAQ-GM-CSF immunoliposomes had better effects on the cytotoxicity of HL60 cells than DHAQ liposomes and DHAQ.The cellar untrastructure was markly destroyed and many apoptotic bodies formed after the treatment. Conclusion:DHAQ-GM-CSF immunoliposomes are successfully prepared and verified,which have targeting cytotoxicity on HL60 cells.The study establishes the foundation for futher investigation.
