Effect of inhibiting mdr1 on radiosensitivity in nasopharyngeal carcinoma cell line
- VernacularTitle:反义mdr1对鼻咽癌细胞放射敏感性的影响
- Author:
Qinglan REN
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal carcinoma cell line;
mdr1;
Radiosensitivity
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of inhibiting mdr1 by antisense technology on radiosensitivity in nasopharyngeal carcinoma cell line(CNE). Methods:There were four groups in the study:control group,lipofectin group,sense oligodexynucleotides(SODN) group,and antisense oligodexynucleotides(ASODN) group. The expression of mdr1 was detected by RT-PCR. Cellular response to irradiation was evaluated by the colony forming test. Methylguanine-DNA methyltransferase(MGMT)protein expression was determined by immunohistochemical staining. Results:ASODN group could significantly decrease the proliferation rate and downregulate the expression of MGMT of irradiated nasopharyngeal carcinoma cells(vs lipofectin group or SODN group,P
