HPLC fingerprint of Stellera chamaejasme of Inner Mongolia Region
- VernacularTitle:内蒙古地区瑞香狼毒药材HPLC指纹图谱研究
- Author:
Zhaolian ZHUO
;
Ying GAO
;
Weimin LI
;
Weidong GAO
;
Xiaohua YAO
- Publication Type:Journal Article
- Keywords:
Stellera chamaejasme;
fingerprint;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a HPLC fingerprint of Stellera chamaejasme L. from Inner Mongolia Region. METHODS: The RP-HPLC method was used with Akzonobel Kromasil C_ 18 (250 mm?4.6 mm, 5 ?m) the acetonitrile-0.5% phosphoric acid (gradient elution) was used as mobile phase, analytic time was 60 min, and detective wavelength was at 297 nm, the column temperature of 15℃ were adopted. RESULTS: The HPLC fingerprint of Stellera chamaejasme L. set up showed that 14 peaks were co-possessing in different sources. The results of method validation met technical standard of fingerprint, the similarities of Stellera chamaejasme L. were 0.9 to 1.0. CONCLUSION: The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of Stellera chamaejasme L. from Inner Mongolia Region.
