Determination of notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 in Zidan Huoxue Tablet by HPLC gradient elution
- VernacularTitle:HPLC梯度洗脱法同时测定紫丹活血片中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1含量
- Author:
Jing CHENG
;
Qun MEI
;
Jixiang HE
;
Hongyun GUO
- Publication Type:Journal Article
- Keywords:
Zidan Huoxue Tablet;
notoginsenoside R_1 and ginsenoside Rg_1, Rb_1;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine notoginsenoside R_1 and ginsenoside Rg_1, Rb_1 in Zidan Huoxue Tablet by HPLC linear gradient elution (total saponins of Radix et Rhizoma Notoginseng). METHODS: Using HPLC method with Hypersil ODS2 C_ 18 column. A mobile phase consisted of acetonitrile-water 0-20 min (20∶80), 20-21 min(40-20∶60-80), 21-30 min(20∶80) gradient elution. The column temperature was at room temperature. The detection wavelength was at 203 nm. RESULTS: The linear ranges of R1, Rg1, Rb1 were 0.52-2.6 ?g (r= 0.999 9 ), 2.106 -10.53 ?g (r= 0.999 6 ) and 2.946-14.73 ?g (r= 0.999 6 ), respectively. The average recoveryies were 99.3% (RSD=1.1%), 100.0%(RSD=0.5%) and 99.7%(RSD=0.9%), respectively. CONCLUSION: The method is selective and reproducible for determination of notoginsenoside R_1 and ginsenoside Rg_1, Rb_1 in Zidan Huoxue Tablet.
