Quality standard for Biling Weitong Granules
- VernacularTitle:荜铃胃痛颗粒的质量标准研究
- Author:
Shenjuan ZHAO
;
Xinlan HUANG
;
Xuelan LI
;
Jingping SHI
- Publication Type:Journal Article
- Keywords:
Biling Weitong Granules;
quality standard;
TLC;
RP-HPLC;
berberine hydrochloride
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish the quality standard of Biling Weitong Granules (Rhizoma Corydalis, Radix et Rhizoma Rhei, Rhizoma Coptidis, etc.). METHODS: Rhizoma Corydalis, Radix et Rhizoma Rhei, Rhizoma Coptidis, Fructus Evodiae, Fructus Litseae were identified by TLC. The content of berberine hydrochloride was determined by RP-HPLC C_ 18 column(ODS, 250 mm?4.6 mm, 5 ?m) was used as chromatographic column. The acetonitrile-0.02 mol/L potassium dihydrogen phosphate (Adjust with phosphoric acid to a pH of 3.0) (25∶75) was used as mobile phase. The detection wavelength was at 347 nm. The flow rate was kept to 1.0 mL/min. RESULTS: The TLC sports developed were fairly clear, and the blank test showed no interference. The linearity of berberine hydrochloride was good in the range of 0.041 32-0.619 8 ?g(r= 0.999 9 ). The average recovery of berberine hydrochloride was 101.93%, RSD=0.60%(n=6). CONCLUSION: The method is simple, reliable, accurate and can be applied as the quantity control method of Biling Weitong Granules.
