Effect of Damp-heat Factor on the Expression of Aquaporin 3,4 Gene in Gastric Mucosa of Rats
- VernacularTitle:湿热因素对大鼠胃黏膜水通道蛋白3、4基因表达的影响
- Author:
Song WEI
;
Shaoxian LAO
;
Zhixin HUANG
- Publication Type:Journal Article
- Keywords:
damp-heat syndrome;
aquaporin;
animal model;
gastric mucosa
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the animal model of damp-heat syndrome(DHS) and explore the expression of aquaporin(AQP) 3,4 gene in gastric mucosa.Methods Thirty SD rats were randomized into 3 groups and observed for 20 days.Group A(normal group,NG) was fed with routine method.Group B(Pi deficiency syndrome group,PDS) was fed alternatively with routine method and aqua of cassia angustifolia Vahl.Group C(DHS) was fed with fat,honey liquid and treated with damp-heat environment.The expression of AQP 3,4 gene in the 3 groups were determined by fluorescence quantitative polymerase chain reaction(FQ-PCR).Results The symptoms and signs in Group C were simiar to the clinical DHS.Expression of AQP3 gene in DHS was statistically significant higher than that in PDS and NG(P
