Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Kangliu Pills by HPLC
- VernacularTitle:HPLC法测定抗瘤丸中三七皂苷R_1及人参皂苷Rg_1、Rb_1的含量
- Author:
Li ZHANG
;
Qian SHEN
;
Yanchuan WU
- Publication Type:Journal Article
- Keywords:
Kangliu pills;
notoginsenoside R1;
ginsenoside Rg1;
ginsenoside Rb1;
HPLC;
determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a HPLC method for the determination of the contents of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Kangliu pills.Methods It was performed on Kromasil C18(4.6 mm?150 mm,5 ?m),and the mobile phase was acetonitrile-0.05% phosphoric acid with gradient elution system.The flow rate was 1.0 mL/min,the detective wavelength was 203 nm,injection volume was 10 ?L and the column temperature was 30 ℃.Results The liner range of notoginsenoside R1 was 82.8～621 ?g(r=0.999 9) and the average recovery was 100.057%(RSD=0.399 6%).The liner range of ginsenoside Rg1 was 109.8～ 823.5 ?g(r =0.999 6) and the average recovery was 99.248%(RSD=1.004 6%).The liner range of ginsenoside Rb1 was 112.6～844.5 ?g(r=0.999 9) and the average recovery was 99.812%(RSD=1.474 4%).Conclusion The method is simple,accurate and reproducible,and suitable for the content determination of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Kangliu pills.
