Determination of relative molecular weight of polysaccharides in Bletilla striata by MALDI-TOF-MS
- VernacularTitle:MALDI-TOF-MS测定天然白芨多糖相对分子质量的研究
- Author:
Anyuan CHENG
;
Zhaojing ZHU
;
Dan XU
- Publication Type:Journal Article
- Keywords:
polysaccharides in Bletilla striata (Thunb.) Reichb. f.;
matrix-assisted laser desorption ionizatior/time-of-flight mass (MALDI-TOF-MS);
matrix;
relative molecular weight
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relative molecular weight of polysaccharides in Bletilla striata by matrix-assisted laser desorption ionizatior/time-of-flight mass (MALDI-TOF-MS). Methods 2, 5-Dihydroxybenzoic acid (DHB) as a matrix was used. By drop drying at room temperature the proportion of the matrix and polysaccharide sample was 1.5∶1. Baseline mode and laser intensity at 1 500-2 500 units were carried out. Results The relative molecular weights of polysaccharides in B. striata were 6?104-3?105, whose distribution rang was wide. Conclusion The relative molecular weights of polysaccharides in B. striata are obtained by MALDI-TOF-MS technique in the proportion of DHB-polysaccharides in B. striata 1.5∶1, which is valuable for the preparation and good quality by the direct drop drying method.
