Biotransformation of furannoligularenone by cell suspension cultures of Nicotiana tabacum
- VernacularTitle:烟草悬浮培养细胞对呋喃橐吾酮的生物转化研究
- Author:
Chunyan YAN
;
Rongmin YU
;
Huachong L
;
Dezhi ZHANG
- Publication Type:Journal Article
- Keywords:
Nicotiana tabacum L.;
suspension cultures;
biotransformation;
furannoligularenone
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biotransformation of furannoligularenone by cell suspension cultures of Nicotiana tabacum. Methods Furannoligularenone was added to the medium of the suspension cells of N. tabacum after precultured for 10 d, then they were co-cultured for another 4 d. The biotransformed products were detected with HPLC and isolated by various chromatographic methods. The chemical structures of biotransformed products were elucidated on the basis of their physicochemical pro-perties and spectroscopic data. Otherwise, the influence of co-cultured time on conversion ratio was investigated either. Results The substrate, furannoligularenone (Ⅰ), was successfully biotransformed by N. tabacum cultured cells. Two products, 3-oxo-eremophila-1, 7(11)-dien-12, 8-olide (Ⅱ) and 3-oxo-8-hydroxyeremophila-1, 7(11)-dien-12, 8-olide (Ⅲ) were obtained. After co-cultured for 5 d, the mole conversion ratio of the substrate reached the highest (53.1%). Conclusion It's the first report on biotransformation of furannoligularenone by N. tabacum cultured cells.
