Quality evaluation of Semen Cassiae by both indicated component determination and HPLC fingerprint
- VernacularTitle:基于指标成分和HPLC指纹图谱检测的决明子化学品质评价研究
- Author:
Jingbin WANG
;
Xu ZHOU
;
Zhifang HU
;
Yongshen REN
;
Xiaohe XIAO
- Publication Type:Journal Article
- Keywords:
Semen Cassiae;
chrysophanic;
quantitative determination;
HPLC fingerprints
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the quality of Semen Cassiae from different habitats objectively. Methods To determine the content of chryso-phanic according to ChP and establish HPLC fingerprints with the gradient elution solvent composed of acetonitrile and 1% HAC. A C18 column (250 mm?4.6 mm, 5 ?m) was used, flow rate: 1 mL/min, detecting wavelength: 254 nm, and the column temperature: room temperature. The clustering analysis was carried out by SAS software according to the content of chrysophanic and similarity of HPLC fingerprints obtained by the software of similarity analysis. Results The established HPLC fingerprint has desirable precision, reproducibility, and stability. The content of chrysophanic and HPLC fingerprints of Semen Cassiae from various habitats are different, which differs from the habitats. The content range of chrysophanic in Semen Cassiae is 0.037%-0.170% and the similarity is 0.864-0.962. Conclusion The method indicates the difference of the chemical component in Semen Cassiae from various habitats and can be used as a quality control method for Semen Cassiae.
