THE EFFECTS OF AECs CONDITIONED MEDIUM ON INDUCING NEURONAL DIFFERENTIATION OF BMSCs
- VernacularTitle:羊膜上皮细胞条件培养液对大鼠骨髓基质细胞神经分化的影响
- Author:
Danni WANG
;
Haimei SUN
;
Rongping LI
;
Juan DU
;
Chunli ZHAO
;
Deyu ZHENG
;
Fengqing JI
;
Hui YANG
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Amniotic epithelial cells;
Inducing;
Differentiation;
Dopaminergic neurons;
Flow cytometry;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of amniotic epithelial cells conditioned medium on the differentiation of bone marrow stromal cells into neural cells. Methods Bone marrow stromal cells and amniotic epithelial cells were isolated and cultured in vitro,then the cell surface antigen was detected by flow cytometry and the expressions of nestin and ki67 were detected by immunofluorescence staining method.When the cells were co-cultured with amniotic epithelial cells conditioned medium,the morphological character of cells was observed by inverse phase-contrast microscope,and the expressions of NSE(neurone specific enolase),TH(tyrosine hydroxylase) and DAT(dopamine transporter) were detected by immunofluorescence staining method. Results Amniotic epithelial cells conditioned medium had obvious inductive effect on bone marrow stromal cell's neural differentiation.Conclusion The amniotic epithelial cells conditioned medium may have inductive effect neuron-like cell's differentiation and dopaminergic neuron-like cell's differentiation of bone marrow stromal cells in vitro.
