Study on p16 methylation status of BEP-2D cells during its malignant transformation by cigarette smoke condensate(CSC)
- VernacularTitle:烟草悬凝物诱导永生化人支气管上皮细胞恶性转化过程中p16基因甲基化改变的研究
- Author:
Cheng ZHANG
- Publication Type:Journal Article
- Keywords:
Cigarette Smoke Condensate;
p16;
Methylation;
Nested-MSP
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study p16 methylation of BEP-2D cells during its malignant transformation.Methods:Normal BEP-2D cells and BEP-2D cells treated by cigarette smoke condensate(CSC) for 15 weeks(W-15),25 weeks(W-25),38 weeks(W-38) and 43 weeks(W-43)respectively were chosen to study the p16 methylation status by Nested methylation specific PCR (Nested-MSP).Results:The p16 methylation was found in W-15,W-25,W-38 and W-43 BEP-2D cells,but not in the normal BEP-2D cell.Conclusion:The p16 methylation may be considered as a kind of biology-marker of the early diagnosis of lung cancer because the p16 methylation occurs in the earlier period of lung cancer.
