Effbct of silencing PAR1 by RNA interference on invasive potential of human colon cancer cell lines
- VernacularTitle:siRNA沉默PAR1基因表达对大肠癌细胞侵袭力的影响
- Author:
Xinyang LU
- Publication Type:Journal Article
- Keywords:
Colon cancer;
RNAi;
Protease-activated receptors;
Invasive
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of RNA interference(RNAi) on PAR1 gene mRNA expression and the invasive petential of human colon cancer cell lines.Methods:Chemically synthesizeddouble stranded RNA(dsRNA) targeting PAR1 was transfect- ed into human colon cancer cell Lovo using SiPORT Lipid.The transfection eficiency was observed under fluorescence confocal microscopy.Expression of PAR1 mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.Cell penetrate matrigel capacity were determined by invitro experiment.Results:PAR1-siRNA effectively in- hibited PAR1 mRNA and protion expression(P
