Determination of serum?-fetoprotein by TRFIA,RIA and ELISA
- VernacularTitle:3种不同方法检测甲胎蛋白的比较
- Author:
Haiwei CHEN
- Publication Type:Journal Article
- Keywords:
Alpha-fetoproteins;
Time-resolved fluoroimmuno assay;
Radio-immuno assay;
Enzme-linked immunoabsorbent assay
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the efficacy of time-resolved fluoroimmuno assay(TRFIA) for quantative determination of?-fetoprotein(AFP) in comparison with radio immuno assay(RIA) and enzme linked immunoabsorbent assay(ELISA). Methods:Thirty serum samples from randomly selected patients were collected to test AFP content with TRFIA,RIA and ELISA respectively.Results:The linear range of AFP was 1 to l 000 ng/ml for TRFIA,5～400ng/ml for RIA and 5～300ng/ml for ELISA.The intraassay coefficients of variation(CV) and interassay CV were 3.3% and 4.7% for TRFIA,5.3% and 8.9% for RIA,and 10.5% and 15.2% for ELISA,respectively.Correlation tests revealed that the results of RIA were more closely correlated with those of TRFIA than with those of ELISA.Conclusion:TRFIA is shown to be a better method for AFP quantitation than RIA and ELISA in terms of precision and linear range.
