Investigation on west Nile virus infection in equine of Yili, Xinjiang uygur autonomous region
- VernacularTitle:新疆伊犁地区马的西尼罗病毒感染情况调查
- Author:
Hong ZHAI
- Publication Type:Journal Article
- Keywords:
West Nile virus;
Real-time RT-PCR;
One step;
Taqman probe
- From:
Journal of Chongqing Medical University
2008;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the epidemic of west Nile virus(WNV) infection in Yili, Xinjiang uygur autonomous region. Methods:One step real-time RT-PCR was performed to detect the west nile virus envelope(WNV E) in peripheral blood mononuclear cells(PBMCs) of 120 equines. Gene sequence and amino acid sequence were analyzed for positive products. Results:No positive product was found in all the samples. Conclusion:No evidence was found to support WNV infection in Yili, Xinjiang uygur autonomous region.
