Research on Nipah virus infection in equine of Yili, Xinjiang Uygur Autonomous Region
- VernacularTitle:新疆伊犁地区马的尼帕病毒感染情况研究
- Author:
Yanxi LIU
- Publication Type:Journal Article
- Keywords:
Nipah virus;
Real-time RT-PCR;
One step;
Taqman probe
- From:
Journal of Chongqing Medical University
2008;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the epidemics of Nipah virus(NiV)in Yili, Xinjiang Uygur Autonomous Region. Methods:One step real time reverse transcriptase polymerase chain reaction(real-time RT-PCR)was performed to detect the NiV N in peripheral blood mononuclear cells from 120 equines. Gene sequence and amino acid sequence were analyzed for positive products.Results:The positive rate of NiV N in PBMCs from 120 equines was 0%(0/120).Conclusion:The investigation do not support that NiV infection exist in Yili, Xinjiang Uygur Autonomous Region.
