HPLC simultaneous determination of peoniflorin,ferulaic acid and paeonol in Shi'er Wuji Baifeng Pills
- VernacularTitle:HPLC同时测定十二乌鸡白凤丸中芍药苷、阿魏酸和丹皮酚含量
- Author:
Xiaomin MAO
;
Xiaobo ZHANG
;
Xiaoqing CHEN
;
Wenbo ZHANG
;
Liping WU
- Publication Type:Journal Article
- Keywords:
Shi'er Wuji Baifeng Pills;
HPLC;
peoniflorin;
ferulaic acid;
paeonol
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method for determining the contents of peoniflorin,ferulaic acid and paeonol in Shi'er Wuji Baifeng Pills(Gallus Domesticus,Radix Astragali,Rhizoma Dioscoreae,Radix Codonopsis,etc.) METHODS: The analytical column was an ODS column.The mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution using a gradient elution.The detection wavelengths of peoniflorin,ferulaic acid and paeonol were at 230,320 and 274 nm,respectively. RESULTS: The calibration curves of peoniflorin,ferulaic acid and paeonol showed good linearity at the ranges of 21.19-211.90,1.01-10.06,10.63-106.30 ?g/mL and the average recoveries were 98.63%,98.35%,99.12%,respectively. CONCLUSION: The method is simple,rapid,reliable and can be used for the drug control.
