Isolation and culture of bone marrow mesenchymal stem cells and the study of their biological char-acteristics in patients with rheumatoid arthritis
- VernacularTitle:类风湿关节炎骨髓间充质干细胞的体外培养和一般生物学特性的初步研究
- Author:
Lihui MA
;
Zhenhua QIAO
;
Xiaofeng LI
;
Liyun ZHANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Mesenchymal stem cell
- From:
Chinese Journal of Rheumatology
2008;12(10):680-683,插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and culture bone marrow mesenchymal stem cells (BMSCs) from patients with rheumatoid arthritis (RA) and examine their biological characteristics. Methods MSCs were isolated from bone marrow of RA patients and purified by density gradient centrifugation and cultured in vitro. The morphology, immunophenotype, and proliferative; property of BMSC and colony forming unit-fibroblast (CFU-F) were measured and analyzed. Results The culture expanded cells from RA patients presented a typical fibroblast-like morphology. Ceils were positive for SH2 (CD105), CD71, and CD44, but negative for CD45. Their proliferative capacity and CFU-F number were similar to those of BMSCs from healthy donors. Conclusion In respect to morphology, immuno-phenotype, proliferative property and colony forming unit-fibroblast (CFU-F), MSCs from bone marrow of RA patients are not different from those of MSCs isolated from bone marrow of normal donors. MSCs from the bone marrow of RA patients have the potential for clinical application.
