Assay of ginsenoside Rb_1,ginsenoside Rg_1 and notoginsenoside R_1 in Sanxue Mingmu Tablet
- VernacularTitle:散血明目片中人参皂苷Rb_1、人参皂苷Rg_1及三七皂苷R_1的含量测定
- Author:
Xinpei ZHOU
;
Rong OUYANG
;
Guiming DENG
- Publication Type:Journal Article
- Keywords:
Sanxue Mingmu Tablet;
ginsenoside Rb_1;
ginsenoside Rg_1;
notoginsenoside R_1;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method for determining contents of ginsenoside Rb_1,ginsenoside Rg_1 and notoginsenoside R_1 in Sanxue Mingmu Tablet(Radix et Rhizoma Notoginseng,Pheretima,Herba Leonuri,Rhizoma Imperatae,etc.). METHODS: The contents of ginsenoside Rb_1,ginsenoside Rg_1 and notoginsenoside R_1 were separated by gradient elution on Hypersil ODS C_(18) column(5 ?m ? 4.6 mm?100 mm) as stationary phase;acetonitrile and water as gradient mixed mobile phase;detected at 203 nm wavelength;(25 ?C) of column temperature and 1.0 mL/min of mobile rate. RESULTS: Ginsenoside Rb_1,ginsenoside Rg_1 and notoginsenoside R_1 had good linearities(r=0.999 9;n=6) at the ranges of 1.480 0-14.800 ng;0.372 0-3.720 ng and 1.072 0-10.720 ng.The average recoveries were 98.44%(RSD=0.96%);99.02%(RSD=0.94%);97.95%(RSD=(1.02%)),respectively. CONCLUSION: This method is high in column efficiency and satisfactory for isolation and repeatability,the result is accurate.It can be adopted for quality control of Sanxue Mingmu Tablet.
