Quantitative Detemination of AGP by DNS Method
- VernacularTitle:3,5-二硝基水杨酸比色法测定红毛五加中多糖的含量
- Author:
Lihua ZHANG
;
Liping ZHANG
;
Jinqun HU
- Publication Type:Journal Article
- Keywords:
Acanthopanax giraldii Harms polysaccharides (AGP);
glucose;
DNS method
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the quantitative determination of AGP (Acanthopanax giraldii Harms polysaccharides). Methods AGP content was determined by DNS Method. The absorptions of processed samples were tested at the wavelength of 520 nm. Results Contrast to glucose, the linear range was 46.6～233.0 mg/L (r=0.999 2), the average recovery was 99.0%～102.1%, RSD=3.52%, n=5. The polysaccharides measured in 3 batches of samples were 1.3～1.9 mg/g. Conclusion The method is sensitive,steady with good repeatability, and help to the qulity control of Acanthopanax giraldii Harms.
