Determination of Chlorogenic Acid, Baicaliin, Forsythin and Wogonin in Shuanghuanglian Oral Liquid by HPLC
- VernacularTitle:高效液相色谱法测定双黄连口服液中绿原酸、黄芩苷、连翘苷、汉黄芩苷的含量
- Author:
Jingqun HUANG
;
Juan YANG
;
Hong LI
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian oral liquid;
chlorogenic acid;
baicaliin;
forsythin;
wogonin;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of chlorogenic acid, baicaliin, forsythin and wogonin in Shuanghuanglian oral liquid. Methods The column was VP-ODS C18 (250 mm?4.6 mm, 5 ?m). The mobile phase consisted of acetonitrile-0.2% phosphoric acid with gradient elution. The flow rate was 1.0 mL/min and the detection wavelength was at 278 nm. Results The calibration curves were linear within the range of 0.44～6.60 ?g (r=0.999 1) for chlorogenic acid, 0.52～6.18 ?g (r=0.999 1) for baicaliin, 0.20～2.04 ?g (r =0.999 3) for forsythin (r =0.999 3) and 0.13～1.76 ?g (r =0.999 1) for wogonin, respectively. The average recovery of them were 97.0% (RSD=1.1%), 98.98% (RSD=1.1%), 103.55% (RSD = 1.1%) and 96.49% (RSD = 1.1%), respectively. Conclusion The method is simple, practicable, accurate and rapid. It can be applied to content determination of chlorogenic acid, baicaliin, forsythin and wogonin in Shuanghuanglian oral liquid.
