Content Determination of Berberine in Zhenjing San by HPLC
- VernacularTitle:HPLC法测定镇惊散中盐酸小檗碱的含量
- Author:
Hongyan WANG
;
Limin YANG
- Publication Type:Journal Article
- Keywords:
Zhenjing San;
Berberine;
HPLC;
contant determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2008;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of content determination of Berberine in Zhenjing San. Method The analysis was carried out on C18 column (4.6 mm?250 mm, 5 ?m) by HPLC. The mobile please was acetonitrile-0.033 mol/L KH2PO4 (22∶78), the flow rate was 0.800 mL/min and detective wave length was 345 nm. Result The cabibration curves showed good linearity in the range of 12.012～600.600 ?g. The average recovery was 98.85% (RSD=1.09%). Conclusion The method is good sensitive, accurate and reliable.
