The cloning,expression and pruification of recombinant
- VernacularTitle:重组人肝脏增生因子克隆表达纯化及生物活性的探讨
- Author:
Xiaobo ZENG
;
Xianshi SU
- Publication Type:Journal Article
- Keywords:
Human augmenter of Iiver regeneration;
Cloning,molecular;
Expression;
Purification
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(11):1838-1840,插三
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide a technique of preparation recombinant hALR by gene engineering in larger scale.Methods Construct hALR(human augmenter of liver regeneration) protein expression vector pGEX-3X-hALR,induce the expreesion of hALR,and pruified the protein by affinity chromatography.Results Succeed in constructing the vector pGEX-3X-hALR and obtained the recombinant hALR protein.Conclusion The mothed could be used for preparation recombinant hALR by gene engineering in larger scale.
