Biotransformation of podophyllotoxin by cell suspension and root culture systems of Rheum palmatum
- VernacularTitle:掌叶大黄细胞悬浮系统和根培养系统对鬼臼毒素的生物转化研究
- Author:
Shihai YANG
;
Jiayuan LIU
;
Bo YU
;
Xiaofeng LIU
- Publication Type:Journal Article
- Keywords:
Rheum palmatum L.;
cell suspension culture system;
root culture system;
podophyllotoxin;
picropodophyllin;
epipodophyllotoxin;
biotransformation
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biotransformation of podophyllotoxin by cell suspension culture and root culture systems of Rheum palmatum.Methods Plant tissue culture technology was employed.The products were isolated by silica gel column chromatography and their structures were elucidated by spectral methods.Results Adding of podophyllotoxin had no obvious effects on the pH value of cell suspension culture and root culture systems of R.palmatum.In the case of present study,73.8% of podophyllotoxin was converted by cell suspension culture and 56.3% by root culture.The products were different between the two culture system.Podophyllotoxin was converted into picropodophyllin with cell suspension culture system and many other products besides epipodophyllotoxin with root culture system.Conclusion Both of cell suspension culture and root culture systems of R.palmatum are able to convert podophyllotoxin.
