Experimental study of matrine to reverse drug-resistance of human multidrug-resistance hepatocellular carcinoma cell
- VernacularTitle:苦参碱逆转人肝癌细胞株QGY/CDDP多药耐药性的实验研究
- Author:
Xiongyang XU
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Multidrug resistance;
Matrine
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the biologic roles and potential reverse mechanisms of matrine in human hepatocellular carcinoma cell line QGY and QGY/CDDP.Methods:The MTT assay was used to detect the IC50s of QGY and QGY/CDDP cells,and QGY/CDDP cell were cultivated with matrine.The distinctions of three groups were compared in cell's appearance,growth curve,doubling time,cell cycle,drug-resistance of chemotherapeutics and CDDP contents of intra-cells.Immunocytochemical method was applied to detect the expression of P-gp,MRP and Bax.Results: Matrine could inhibit the growth of QGY and QGY/CDDP cells with no cross drug-resistance.There were some biological differences among QGY,QGY/CDDP and Mat-QGY/CDDP cells.The expressions of P-gp and MRP were decreased;Bax expression was increased,CDDP's toxic effect on QGY/CDDP cells was enhanced,and the drug sensitivity was elevated in Mat-QGY/CDDP cells.Conclusion:Matrine at lower toxic-dose could enhance the toxicity of CDDP on QGY/CDDP cells,and partly effective in reversing the drug-resistance in human multidrug-resistant hepatocellular carcinoma QGY/CDDP cells.
