Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line(SKOV_3) in nude mice and analysis of its mechanism
- VernacularTitle:二氧化碳气腹对人卵巢癌细胞系SKOV_3裸鼠腹腔转移瘤生长的影响及机制探讨
- Author:
Junying TANG
- Publication Type:Journal Article
- Keywords:
Carbon dioxide pneumoperitoneum;
Nude mice;
Apoptosis;
VEGF;
MMP-9
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of carbon dioxide(CO2) pneumoperitoneum on the abdomino-metastatic tumor growth of ovarian carcinoma cell in nude mice and to primarily analyze its mechanism.Methods:The nude mice model of abdomino-metastatic tumor of ovarian carcinoma cell was established,and laparoscopic CO2 pneumoperitoneum environment was simulated.Tumor implantation and growth were observed and tumor tissue apoptosis was detected by TUNNEL method,to explore the effect of CO2 pneumoperitoneum on the abdomino-metastatic tumor growth of ovarian carcinoma cell in nude mice.The expressions of VEGF and MMP9 in cancer tissue were determined by immunohistochemistry and Western Blot to analyze the possible mechanism.Results:After the treatment of CO2 pneumoperitoneum,the tumor quality in abdominal cavity was higher than that of the control group,the expression difference of VEGF and MMP9 in tumor tissues was not significant and tumor cell apoptosis rates of each group do not differ significantly.Conclusions:Carbon dioxide pneumoperitoneum can promote the growth of ovarian carcinoma cell SKOV3.
