Assay method of nobiliside-A
- VernacularTitle:海参皂苷Nobiliside-A的含量测定方法探讨
- Author:
Yang XIONG
- Publication Type:Journal Article
- Keywords:
Nobiliside-A;
HPLC-ELSD;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish the assay method of Nobiliside-A,an anticancer active component in sea cucumber,by HPLC. METHODS:Comparative experiment was carried out between different detector and different chromatographic column and different mobile phase,at last a HPLC-ELSD method was determined,and linear range,precision and stability were studied. RESULTS:ELSD detector and Agilent Zorbax SB-C_(18) column were used,mobile phase was methonal:0.5%HAC(78(∶)22),the natural logarithm of content of Nobiliside-A and the natural logarithm of the peak area were linear in the range of 0.273～4.24 ?g(r=(0.999 5)),stability and precision within day and between day were satisfied. CONCLUSION:The experiment has provided a rapid and precise method for determining the content of Nobiliside-A.
