Proteromics Analysis and Identification on Peripheral Blood Lymphocyte of Ganyang Huafeng Syndrome of Cerebral Infarction
- VernacularTitle:脑梗死肝阳化风证外周血淋巴细胞相关蛋白质鉴定和分析
- Author:
Nianju ZENG
;
Qinghua LIANG
;
Xingui XIONG
- Publication Type:Journal Article
- Keywords:
Ganyang Huafeng Syndrome;
cerebral infarction;
MALDI-TOF-MS;
proteromics
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and identify the proteins relevant to Ganyang Huafeng Syndrome(GYHF) and Yinxu Shengfeng Syndrome(YXSF) of cerebral infarction,and investigate the essence of GYHF and YXSF.Methods 3 groups,GYHF of cerebral infarction,YXSF of cerebral infarction and healthy people were designed with 15 cases of each group.Total protein were extracted from lymphocyte of peripheral venous blood,and two dimensional electrophoresis was used to separate total proteins from norma land model ratsynovium.Having been stained,proteins were analyzed by PDquest software.Some selected protein spots were identified by peptide mass fingerprint(PMF) based on Matrix assisted laser desorption/ ionizationtime of flight mass spectrometry(MALDI-TOF-MS) and database.Results 23 different protein spots and 8 identical peotein spots were found out from GYHF and YXSF group,22 proteins were identified from the different protein spots including 15 kinds of proteins and 6 protein spots including 5 kinds of proteins.GYHF of cerebral infarction related with proteins such as metabolic enzymes,signal transmission proteins,cytoskeletal protein,antioxydation proteins and so forth.Conclusion GYHF and YXSF of cerebral infarction have different proteins and identical proteins,which indicates that the two syndromes have the same matter substructure and different essential connotation.
