Effects of Qidan Tongmai Tablet on IL-10 in the Rat Model of Myocardial Ischemia/Reperfusion Injury
- VernacularTitle:芪丹通脉片对心肌缺血/再灌注大鼠白细胞介素-10的影响
- Author:
Ye HUANG
;
Zongren WANG
;
Juan XIE
- Publication Type:Journal Article
- Keywords:
Qidan Tongmai Tablet;
myocardial ischemia/reperfusion injury;
inflammatory reaction;
IL-10;
myocardial ultrastructur;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Qi-reinforcing and blood-activating prescription,Qidan Tongmai Tablet(QDTMT) on the IL-10 in the rat model of myocardial ischemia/reperfusion injury.Methods A model of myocardial ischemia/reperfusion injury was reproduced by ligation of left anterior descending branch of the coronary artery.Thirty-six male SD rats were randomly divided into six groups(n=6):sham operation group,model group,Diltiazem Hydrochloride group,QDTMT high-dose group,QDTMT medium-dose group and QDTMT low-dose group.After the left anterior descending branch of coronary artery was occluded for 30 minutes followed by reperfusion for 2 hours,the whole blood was collected via right common carotid artery and the serum level of IL-10 was measured by ELISA.At the same time,HE staining was performed to detect the pathological change in myocardium,and the ultrastructural alterations of rats were observed with transmission electron microscope.Results The contents of IL-10 in serum were increased in QDTMT groups.Electron microscopic examination showed that pathologic changes of myocardiocytes in QDTMT groups were milder than those of the model group.The effects in QDTMT high-dose group were better than that in the low-dose group,and has insignificant difference with Diltiazem Hydrochloride group.Conclusion Qi-reinforcing and blood-activating prescription inhibits the inflammatory reaction by facilitating the expression of endogenous IL-10 and improving myocardial ultrastructural changes after myocardial ischemia/reperfusion in rats.These actions may contribute to its protective effect on the ischemic myocardial cells.
