Determination of Bullatine A in Gutongling Babu Plaster by HPLC
- VernacularTitle:HPLC法测定骨痛灵巴布剂中雪上一枝蒿甲素含量
- Author:
Junhong LI
;
Rixian JIN
;
Bin ZHAO
- Publication Type:Journal Article
- Keywords:
Gutongling;
Babu plaster;
Bullatine A;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of Bullatine A in Gutongling Babu plaster. Method The Bullatine A was determined by HPLC on Kromasil-C18 column, and the mobile phase consisted of phosphate buffered solution (pH 7.3) - methanol (28∶72), the detection wavelength was 210 nm. Result A good linear relationship of Bullatine A was showed in the range of 0.212 0～4.770 0 ?g (r =0.999 99), and the average recovery was 100.10%, RSD=2.53% (n =6). Conclusion The method is accurate and reliable, and can be used for quality control of Gubitong Babu plaster.
