Preparation of neoeriocitrin and naringin reference substances from Drynaria fortunei
- VernacularTitle:从骨碎补中制备新北美圣草苷和柚皮苷对照品的研究
- Author:
Chunhua YUE
- Publication Type:Journal Article
- Keywords:
Drynaria fortunei(Kunze)J.Sm.;
neoeriocitrin;
naringin;
reference substance
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a separation method for neoeriocitrin and naringin reference sub- stances from Drynaria fortunei.Methods The extract of D.fortunei was isolated and purified by macro porous resin,silica gel column chromatography,neutro-aluminum oxide adsorbing,Sephadex LH-20 co- lumn chromatography,and recrystallizations.The structures of neoeriocitrin and naringin were identified by MS,IR,UV,~1H-NMR,and ~(13)C-NMR.Results The contents of neoeriocitrin and naringin were 99.5% and 99.3%,respectively.Conclusion The developed method is simple with lower cost,by which neoeriocitrin and naringin can be used as reference substances for the qualitative and quantitative analysis of Chinese herbal medicine.
