THE ROLE OF Erk SIGNAL PATHWAY IN B104 CM-INDUCED OPC DIFFERENTIATION FROM NSCs
- VernacularTitle:Erk信号途径在B104 CM诱导神经干细胞向少突胶质细胞前体分化中的作用
- Author:
Jianguo HU
- Publication Type:Journal Article
- Keywords:
B104 CM;
NSCs;
OPCs;
Differentiation;
Erk;
Western blotting;
RT-PCR;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of extracellular signal-regulated kinase(Erk) and transcriptional factors such as c-fos,c-jun in B104 CM-induced oligodendrocyte progenitor cells(OPCs) differentiation from neural stem cells(NSCs).Methods The effect of U0126,the specific inhibitor of Erk1/2,on B104 CM-induced OPC differentiation from NSCs was observed by morphology.The phosphorylation of Erk1/2 and mRNA expression of transcriptional factors c-fos,c-jun and c-myc in NSCs in the control group,B104 CM-treated group and U0126 pre-incubated group were detected by Western blotting and RT-PCR methods respectively.Results B104 CM-induced OPC differentiation from NSCs could be blocked by pre-incubation of U0126.Treatment of NSCs with B104 CM produced a rapid increase in phosphorylation of Erk1/2 and an upregulation of c-fos and c-jun mRNA expression,which could be blocked by the pre-incubation of U0126.Conclusion B104 CM instructs oligodendrocyte differentiation from NSCs through the activation of Erk1/2 signal pathway and sequential upregulation of the expression of transcriptional factors c-fos and c-jun.
