THE FUNCTION OF HOXa-10 GENE IN THE COURSE OF MOUSE BLASTOCYST IMPLANTATION
- VernacularTitle:HOXa-10基因在小鼠胚胎着床过程中的作用
- Author:
Shengjian ZHOU
- Publication Type:Journal Article
- Keywords:
HOXa-10;
Gene;
Endometrium;
Blastocyst;
Implantation;
Real-time fluorescence quantitative PCR;
Mouse
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the function of HOXa-10 gene in the course of mouse blastocyst implantation. Methods Real-time fluorescence quantitative PCR was applied to detect the expression of HOXa-10 gene in normal mice and day1,day2,day3 day4,day5 and day7 pregnant mice.Plasmid containing the HOXa-10 cDNA fragment with liposome and plasmid containing HOXa-10 antisense oligonucleotides with liposome were transfected respectively into the uterine horns of day 2 pregnant mice,and the number of blastocyst-implanted was counted and compared with that of normal day 2 pregnant mouse. Results HOXa-10 gene expression in the pregnant group is higher than that of the non-pregnant group,and the results showed a gradual increasing trend as days went by: HOXa-10 gene expression was the highest on pregnancy d4,began descending on pregnancy d5 and was close to that of non-pregnancy on pregnancy d7.The number of blastocyst increased in the uterine horns which were injected with plasmid containing the HOXa-10 cDNA fragment with liposome.On the contrary,the number of blastocyst decreased in the uterine horns which were injected with plasmid containing HOXa-10 antisense oligonucleotides with liposome(P
