THE EXPRESSION OF PANCREATIC TRIGLYCERIDE LIPASE IN THE RAT PRIMARY TYPE-1 AND TYPE-2 ASTROCYTES
- VernacularTitle:胰甘油三酯脂酶在大鼠两型星形胶质细胞中的表达
- Author:
Meijuan YAN
- Publication Type:Journal Article
- Keywords:
Type-1 astrocyte;
Type-2 astrocyte;
Pancreatic triglyceride lipase;
Cell culture;
Reverse transcriptase polymerase chain reaction(RT-PCR);
Immunohistochemistry;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To compare the expression of pancreatic triglyceride lipase in type-1(T1A) and type-2 astrocytes(T2A).Methods Purified cultures of cortical T1A and T2A were prepared from neonatal rats.The expression of pancreatic triglyceride lipase mRNA was detected by reverse transcriptase polymerase chain reaction(RT-PCR).And the protein level was determined by immunocytochemistry with laser scanning confocal microscope.Results RT-PCR showed that the expression of pancreatic triglyceride lipase mRNA was negative in T1A and positive at a high level in T2A.Immunocytochemistry demonstrated that pancreatic triglyceride lipase expressed at a low level in T1A and was mainly distributed in the nuclei.By contrast,pancreatic triglyceride lipase expressed at a high level in T2A and was distributed diffusedly in the cytoplasm,nuclei and processes.Conclusion T1A and T2A showed different expression levels of pancreatic triglyceride lipase. Pancreatic triglyceride lipase expressed at a high level in T2A,which indicated that T2A may play an important role in the metabolism of lipids in the central nervous system.
