EFFECT OF CGRP AND NGF ON THE PHOSPHORYLATION OF CREB PROTEIN IN RAT HIPPOCAMPUS DURING FOCAL CEREBRAL ISCHEMIA/REPERFUSION
- VernacularTitle:CGRP及NGF对局灶性脑缺血再灌注大鼠海马cAMP反应元件结合蛋白磷酸化的影响
- Author:
Zhenghong ZHANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Calcitonin gene related peptide;
Nerve growth factor;
Hippocampus;
c-AMP response element binding protein;
Immunohistochemistry;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the effect of exogenous calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of cyclic AMP response element binding protein(CREB) and phosphorylated CREB(p-CREB) in rat hippocampus during focal cerebral ischemia/reperfusion(I/R). Methods Focal cerebral ischemia/reperfusion model was set up by occlusion of the right middle cerebral artery using the intraluminal suture method.The expression of CREB and p-CREB in the right hippocampus were detected with immunohistochemistrical SABC method and Western blotting.The immunoreactive positive products were analyzed by image analysis system. Results The expression of CREB decreased in I/R group as compared with sham group in right hippocampus,but the expression of p-CREB was higher in I/R group than that in sham group(P
