Metabolism method of dextromethorphan in vitro
- VernacularTitle:右美沙芬的体外代谢方法研究
- Author:
Lili ZHANG
- Publication Type:Journal Article
- Keywords:
Dextromethorphan;
In vitro metabolism;
High performance liquid chromatography
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate in vitro metabolism method and HPLC method for the dextromethorphan.Methods:Total protein of normal liver specimens metabolismed dextromethorphan in vitro.An HPLC method with fluorescence was developed to study the contents of dextromethorphan and metabolites by joining an internal standard(Adriamycin).Methanol-10mmol/l,sodium dihydrogen phosphate solution(50:50,contain 0.01% sodium heptanesulfonate,5% triethylamine,pH 3.5)was used to the mobile phase.Results:Total protein of normal liver specimens metabolismed dextromethorphan in vitro efficiently with coenzyme,Mg~(2+), suitable temperature and pH value.Dextromethorphan and metabolites were analyed by HPLC method with fluorescence.The metabolic rate of dextromethorphan was 16.09%.Conclusion:It is suggested that the metabolism method of dextromethorphan in vitro is simple,convenient and accurate.It can be used for the determination of metabolism dextromethorphan in vitro.
