Preparation and characterization of monoclonal antibody against S protein of HBV
- VernacularTitle:抗HBsAg单克隆抗体的制备与生物学特性的鉴定
- Author:
Xuemei WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Hepatitis B surface antigen(HBsAg);
Monoclonal antibody
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare anti-HBV S protein hybridoma cell line,this will be helpful to the establishment of rapid HBV infection diagnosis assay.Methods:BALB/c mice were immunized with the recombinant Hepatitis B vaccine.A hybridoma cell line which consistently secreted monoclonal antibody(McAb)against HBsAg was obtained through cell fusion.The specificity of the McAb was analysed by indirect ELISA and Western blot analysis.The immunoglobulin(Ig)subtypethe ascites titers, and the affinity of the obtained mAbs were determined by indirect ELISA.Results:From over hundred positive hybridomas which secreted anti-S protein mAbs,one of hybridomas was screened out,designated 4D2.The subtype of the McAb was lgG_1. The titer of 4D2 mAb produced by ascites fluid was over 1:10~6.The relative affinity constant of 4D2 mAb was determined as 10~5.Conclusion:One of high titer,specific mAb against HBV S protein has been successfully prepared and primarily identified,which may be useful in the development of a rapid and convenient diagnostic kit for detection of HBV infection.
