Determination of Emodin, Chrysophanol and Sodium Danshensu in Shen An Kang Capsules by HPLC
- VernacularTitle:HPLC法测定肾安康胶囊中大黄素、大黄酚和丹参素钠的含量
- Author:
Suzhong ZHANG
;
Dongmei SUN
;
Wenhui LUO
- Publication Type:Journal Article
- Keywords:
Shen An Kang Capsules;
Emodin;
Chrysophanol;
Sodium Danshensu;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining the contents of chrysophanol, emodin and sodium Danshensu in Shen An Kang Capsules(SAKC). Methods Chrysophanol and emodin contents were determined on Lichrospher100 C18 column (4.6 mm? 250 mm, 5 ? m) with the mobile phase of Methanol- 0.1 % Perchloric acid (85 ∶ 15) at a flow rate of 1 mL? min-1, and the detection wavelength was 254 nm. Sodium Danshensu content was determined on Kromasil column C18( 4.6 mm? 250 mm, 5 ? m) with the mobile phase of actonenitirile - 1.2 % Glacial acetic acid(8 ∶ 92)at a flow rate of 0.5 mL? min-1, detection wavelength being 280 nm. Results The linearity of emodin was obtained in the range of 0.020 16～ 0.100 80 ? g(r=0.999 6), linearity of chrysophanol obtained in the range of 0.034 56～ 0.172 80 ? g(r=0.999 9)and linearity of sodium Danshensu obtained in the range of 0.144 8～ 0.868 8 ? g(r=0.999 6).The average recovery was 97.70 % with RSD 2.38 % for emodin, 97.69 % with RSD 1.92 % for chrysophanol and 97.52 % with RSD 0.77 % for sodium Danshensu. Conclusion The method is accurate, reproducible , and is helpful for the quality control of Shen An Kang Capsules.
