Neuroprotective effects of tanshinone I from Danshen extract in a mouse model of hypoxia-ischemia.
10.5115/acb.2013.46.3.183
- Author:
Jae Chul LEE
1
;
Joon Ha PARK
;
Ok Kyu PARK
;
In Hye KIM
;
Bing Chun YAN
;
Ji Hyeon AHN
;
Seung Hae KWON
;
Jung Hoon CHOI
;
Jong Dai KIM
;
Moo Ho WON
Author Information
1. Department of Neurobiology, Kangwon National University School of Medicine, Chuncheon, Korea. mhwon@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Hypoxia-ischemia;
Neuronal death;
Neuroprotection;
Radix Salvia miltiorrhiza
- MeSH:
Animals;
Brain;
Brain Infarction;
Diterpenes, Abietane;
Drugs, Chinese Herbal;
Eosine Yellowish-(YS);
Fluoresceins;
Hematoxylin;
Hypoxia-Ischemia, Brain;
Mice;
Neurons;
Neuroprotective Agents;
Risk Factors;
Salvia miltiorrhiza;
Stroke;
Tetrazolium Salts
- From:Anatomy & Cell Biology
2013;46(3):183-190
- CountryRepublic of Korea
- Language:English
-
Abstract:
Hypoxia-ischemia leads to serious neuronal damage in some brain regions and is a strong risk factor for stroke. The aim of this study was to investigate the neuroprotective effect of tanshinone I (TsI) derived from Danshen (Radix Salvia miltiorrhiza root extract) against neuronal damage using a mouse model of cerebral hypoxia-ischemia. Brain infarction and neuronal damage were examined using 2,3,5-triphenyltetrazolium chloride (TTC) staining, hematoxylin and eosin histochemistry, and Fluoro-Jade B histofluorescence. Pre-treatment with TsI (10 mg/kg) was associated with a significant reduction in infarct volume 1 day after hypoxia-ischemia was induced. In addition, TsI protected against hypoxia-ischemia-induced neuronal death in the ipsilateral region. Our present findings suggest that TsI has strong potential for neuroprotection against hypoxic-ischemic damage. These results may be used in research into new anti-stroke medications.
