Effect of RPM on bcl-2 and Fas protein expression in renal ischemia-reperfusion injury of rats
- VernacularTitle:雷帕霉素对大鼠缺血再灌注肾Fas，bcl-2蛋白表达的影响
- Author:
Chuandong ZHENG
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Rapamycin;
Apoptosis;
Fas;
Bcl-2
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of RPM on bcl-2 and Fas protein expressions at different ischemia-reperfusion points.Methods:Acute ischemic renal injury model was established(right kidney ectomized,45 min of left renal ischemia and reperfusion for Oh,24h,48h and 72h).Fifty four normal male Wistar rats were.randomly divided into 3 groups:sham-operated group(sham,without renal ischemia),ischemia-reperfusion group(IR,without RPM),RPM and ischemia-reperfusion group(RPM 4mg/(kg?d)?3d,reperfusion at 2h before,operation and each observation day).Pathomorphological changes of renal ischemia- reperfusion injury were observed by HE stain and SABC immunohistochemical methods were used to detect the changes of expressions of bcl-2 and Fas.Results:bcl-2 and Fas concentration were significantly higher in IR group than those in sham group(P
