Effect of Ligustrazine on the Expression of Connective Tissue Growth Factor and Osteopontin in the Tubulointerstitium of Diabetic Nephropathy Rats
- VernacularTitle:川芎嗪对糖尿病肾病大鼠肾间质结缔组织因子及骨桥蛋白表达的影响
- Author:
Yan YANG
;
Chunguang XIE
;
Yingjie DU
- Publication Type:Journal Article
- Keywords:
Ligustrazine;
Diabetic nephropathy;
Tubulointerstitium;
Connective tissue growth factor;
Osteopontin
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ligustrazine (TMP) on the expression of connective tissue growth factor (CTGF) and osteopontin (OPN) in the renal tubulointerstitium of diabetic nephropathy (DN) rats. Methods Wistar rats were used to establish the models through intraperitoneal injection of onedose streptozotocin (STZ) and nephrectomy. Rats were divided into 5 groups at random: model group, blank control group, Lotensin (1.7 mg? kg- 1? d- 1) group, of high-and lowdosage of TMP groups (150, 50 mg? kg- 1? d- 1). Except the rats in blank group and model group, the rats received corresponding medicines according to the experimental design for 12 weeks.Volume of urine protein within 24 hours was examined in the 4th, the 8th and the 12 th week, OPN mRNA expression in the renal tubulointerstitium was detected by RT- PCR and CTGF was detected by means of immunohistochemistry. Results Compared with normal group, the volume of 24 h urine protein rised obviously (P
