Effect of Qingchang Suppository on the expression of caspase-3 in an experimental model of ulcerative colitis
- VernacularTitle:清肠栓对大鼠溃疡性结肠炎caspase-3影响的研究
- Author:
Jianqun XIE
- Publication Type:Journal Article
- Keywords:
Qingchang Suppository;
ulcerative colitis;
colonic lamina propria;
caspase-3
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the lymphocyte apoptosis of colon mucosal lamina propria and the expression of its regulatory proteins caspase-3 in an experimental models of ulcerative colitis in rats as well as to survey the effect of Qingchang Suppository(Herba Portulacae,Indigo naturalis,Radix et Rhizoma notoginseng,Galla Chinensis) on intestine mucosa in ulcerative colitis. METHODS: 64SD rats were randomly divided into 4 groups,each group consisted of sixteen animals,group(1):normal control;group(2): blank;group(3): SASP solution;group(4): Qingchang Suppository.The rat model of ulcerative colitis was brought about by an enema of 100 mg/kg of TNBS.Beginning from the third day,except for the normal group,each group was treated with an enema of 0.9% NaCl、 SASP、 Qingchang Suppository respectively.The rats were sacrificed 1 week later.Apoptosis of lymphocyte was detected with the technique of transmission electron microscope(H600),While caspase-3 proteins expressions were assayed with immunohistochemical staining、reverse-transcript PCR,Western-blot. RESULTS: Qingchang Suppository group showed higher rates of apoptosis than the blank controls.Rates of caspase-3 expression cells in LP were higher in UC than its expression in the controls(P
