Research on mast cells and degranulation changing in BALB/c mice gastric mucosal induced by helicobacter pylori
- VernacularTitle:幽门螺杆菌致BALB/c小鼠胃粘膜内肥大细胞及其脱颗粒变化的研究
- Author:
Jing LV
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Chronic urticaria;
Mast ceils;
Degranulation
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To search the impact of helicobacler pylori infection in changes of mice gastric mucosa mast cells and degranulation process,therefore to establish the important role of Hp in the pathogenesis of chronic urticaria.Methods:50 BALB/c healthy mice were randomized into two groups:Hp group,consist of 30 mice,were given Hp liquid every 3 days for total 7 times.Bovine albumin serum(BAS)as control group,was given BAS 2% with the same way as Hp group.Toluidine blue staining of the gastric mucosa was used to assess the total number of mast cells,the amount of degranulated mast cells and the percentage of degranulation after feeding.Each slide was cnnseeutive count with 10 high-power fields for mast cells; results express with number of mast cells/high power field,the ratio of degranulated mast cell was expressed by the amount of degranulated mast cells/total of mast cells.At the same time compared with the control group.Results:There was significant statistical difference in the total number of mast cells between the tow groups(100.0vs0.7,P
