Analysis of polysaccharide composition of Hedyotis difusa Willd polysaccarides by pre-column derivatization HPLC
- VernacularTitle:柱前衍生化HPLC分析白花蛇舌草多糖中单糖组成
- Author:
Ludan BAO
;
Guofang XU
;
Zheng MA
;
Na GUO
;
Xingjie GUO
- Publication Type:Journal Article
- Keywords:
Hedyotis difusa Willd polysaccharide;
pre-column derivatization;
high performance liquid chromatogaphy;
monosaccharide
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:The polysaccharides of Hedyotis difusa Willd of different sources were extracted and its content and composition of the polysaccharide was analyzed. METHODS: The microwave was employed for the aqueous extract of Hedyotis difusa Willd and the content of total sugar was determined by phenol-sulfur test,the monosaccharides composition was determined by HPLC with pre-column derivatization. RESULTS: The polysaccharide obtained from the samples of Guangdong Province had the highest content.The analytic data showed that the composition of monosaccharides in hydrolytic polysaccharides of Hedyotis difusa Willd were mannose,glucose,galactose,arabinose and rhamnose,the average content of polysaccharides was 9.82%. CONCLUSION: The HPLC method with pre-column derivatization is appropriate for the analysis of monosaccharide composition of Hedyotis difusa Willd polysaccharides and the method is simple,quick and accurate.
